The study involves the growth and maintenance of Mycoplasma pneumoniae and Pseudomonas aeruginosa in various media and conditions. The researchers prepared liquid Hayflick complete medium and solid Hayflick 1% agar plates for M. pneumoniae growth. They also used Tryptic Soy Broth (TSB) medium for P. aeruginosa growth. The study includes the generation of M. pneumoniae mutant strains, plasmid construction, and protein quantification using mass spectrometry.
The researchers performed in vitro biofilm degradation assays and antimicrobial activity tests. They also conducted mouse experiments to study the efficacy of M. pneumoniae in preventing P. aeruginosa infections. The mice were inoculated with M. pneumoniae and P. aeruginosa, and their lungs were analyzed for histopathological changes and RNA extraction.
The study also involved the collection of endotracheal tubes (ETTs) from patients with P. aeruginosa infections and the analysis of P. aeruginosa dispersal from the ETTs. The researchers used various statistical methods to analyze the data, including one-way ANOVA, Kruskal-Wallis test, and Spearman’s correlation analysis. The study aims to investigate the potential of M. pneumoniae as a therapeutic agent against P. aeruginosa infections.