Researchers at Fujita Health University and Nara Institute of Science and Technology have developed a hybrid ‘in silico/in-cell’ controller (HISICC) to regulate the production of fatty acids in Escherichia coli (E. coli) bacteria. The HISICC combines a computer-driven, model-based optimization controller with a feedback control mechanism engineered directly into the bacteria. This approach allows for the regulation of the key enzyme acetyl-CoA carboxylase (ACC) to improve fatty acid yield and reduce losses due to process-model mismatches (PMMs). The team tested three different control strategies, including a traditional “no brakes” approach and two new approaches using the HISICC. The results showed that the HISICC approach achieved the highest fatty acid yield. The researchers believe that this technology has the potential to improve the production efficiency of fuels and important chemicals, reducing costs and environmental impact. The study was funded by the Next Generation Interdisciplinary Research Project and AMED. The authors declare no competing interests.
A cutting-edge hybrid controller has the potential to maximize efficiency.
