Silver nanoparticles (AgNPs) were synthesized using the cell-free supernatant of the thermophilic bacterium Geobacillus stearothermophilus GF16. The formation of AgNPs was verified by a color change and UV-Vis spectroscopy. The effects of temperature, pH, incubation time, and AgNO₃ concentration on AgNP formation were studied. The results showed that higher temperatures and precursor concentrations led to the formation of larger nanoparticles. The AgNPs were characterized using DLS, FTIR, and electron microscopy, revealing a subspherical shape and a biomolecular coating. The nanoparticles exhibited thermal stability, antioxidant activity, and antimicrobial efficacy against various microorganisms. The AgNPs also showed catalytic efficiency in the degradation of toxic compounds, such as Congo Red and 4-nitrophenol. The study highlights the potential of AgNPs synthesized using thermophilic bacteria for various applications, including biomedical, environmental, and industrial fields. The results demonstrate the importance of optimizing synthesis parameters to tailor the properties of AgNPs for specific applications. Overall, the study provides valuable insights into the design and optimization of nanoparticle synthesis protocols for diverse applications.
Biosynthesis of antimicrobial and antioxidant silver nanoparticles using thermophilic bacteria G. stearothermophilus GF16 yields stable nanomaterials with catalytic properties.
