Here is a summary of the content within the 200-word limit:
The diversity of biochemical systems in nature has led to the development of various valuable compounds, including secondary metabolites that are of great importance to industries. However, the range of compounds that can be generated through biosynthesis is limited to those that occur naturally in living systems and have known biosynthesis pathways. To diversify natural metabolic pathways and produce novel valuable compounds, researchers have employed various strategies.
One approach is to rewrite metabolic blueprints using natural enzymes, which can be achieved through gap-filling and mix-and-matching of pathways. This can lead to the production of novel compounds, such as odd-chain fatty alcohols and arbutin. Another approach is to design non-natural metabolic pathways using computational tools, which can enable the production of novel compounds such as 2,4-dihydroxybutyric acid.
To achieve this, researchers have used various strategies, including the introduction of non-native enzymes, the creation of artificial enzyme channels, and the manipulation of pathways to produce novel compounds. These approaches have led to the production of various valuable compounds, including salvia, erythromycin, and 2-pyrrolidone.
Overall, the diversification of natural metabolic pathways is crucial for the production of novel valuable compounds, and the development of novel strategies and technologies is necessary to achieve this goal.